Tooth Enamel- Composition, Properties, Structure and Functions

Tooth Enamel


Tooth Enamel is an ectodermal derivative and is the most highly mineralized tissue known. 

It is the hardest tissue of the body, which covers entire surface of the anatomical crown of all the teeth. 
Tooth Enamel
Tooth Enamel


Enamel provides shape and contour to the crown of teeth. It is also known as Substantia adamantia.



1) Physical Properties

A) Thickness- Enamel is thicker in the cusp of the molars and premolars, reaching up to a maximum of 2–2.5 mm, whereas at the cervical region, it almost thins down to a knife edge.

B) Hardness and Density- Varies in different parts of the crown. The hardness and density decreases from the surface of the enamel to the dentinoenamel junction (DEJ) and from the cuspal or incisal region to the cervical margin.

C) Specific Gravity- The specific gravity of enamel is 2.8

D) Color- The colour of the enamel varies from light yellow to greyish white. The colour depends on the translucency of the enamel, which is mostly associated with a variation in the degree of calcification and homogeneity of the enamel. The refractive index of enamel is 1.62.

E) Permeability- Enamel acts like a semipermeable membrane, allowing complete or partial passage of certain molecules. The permeability of the enamel is the result of the presence of cracks and microscopic spaces on the surface of enamel which allow the penetration of fluids.

F) Brittle- The structure and hardness of the enamel makes it brittle.

G) Density- The density of enamel is 2.8 to 3 g/ml. 

H) KHN- It has a Knoop hardness number (KHN) of 343 while dentin has 68 KHN and cementum has 40 KHN. Enamel resists masticatory impact of about 10 to 20 kg per tooth.


2) Chemical Properties

The enamel is made up of 96% inorganic material and 4% organic matter and water by weight. 

The inorganic content is mainly hydroxyapatite crystal and is present 92 to 98 percent by volume of total inorganic matter

The organic matrix of enamel is made up of two types of non-collagenous protein components: amelogenins (90%) and non-amelogenins (10%). 

The non- amelogenins constitute the enamel proteins enamelin, ameloblastin, sulphated protein and tuftelin.


  • Read About
  1. Dentin- Microscopic Structure, Properties, Types and Functions
  2. Dental Pulp- Morphology, Histology, Structure and Function



3) Structure of Enamel

Enamel is composed of the following.

A. Enamel rods (prisms)
B. Rod sheaths
C. Inter rod substance (cement)


A. Enamel Rods (Prisms)

Structurally enamel is composed of millions of enamel rods or prisms.


Enamel Rods, Enamel Prisms, Hydroxyapatite crystals

An enamel rod is a long, thin structure extending from the dentino-enamel junction to the 
surface of enamel.


enamel rods




Follows a tortuous course; thus the length of an enamel rod may be greater than the 

thickness of enamel.

Each rod is formed by four ameloblasts. One ameloblast forms the rod head, a part of two 
ameloblasts form the neck, and the tail is formed by a fourth ameloblast. Each ameloblast 
contributes to four different rods.

In a cross-section of human enamel, many rods resemble fish-scales.

fish scale structure of tooth enamel, enamel rods fish scale pattern


Direction of rods In general, the rods are directed at right angles to the dentino-enamel  
junction and the tooth surface. 

In cervical regions of deciduous and permanent  teeth,  the  directions of enamel rods are 
different

In permanent teeth: At the central part of the fossa and pits, the rods turn oblique to nearly 
horizontal. 
Near the tip of the cusp and incisal edge, the rods gradually change to increasingly oblique 
direction. 
Gradually they become nearly vertical at the tip of the cusp 
and incisal region. 
In the cervical region, the rods run slightly apically from dentin surface to outer enamel 
surface


enamel rods direction in deciduous and permanent teeth
In deciduous teeth: The arrangement of rods in the occlusal two-thirds in deciduous teeth 
is similar to that in permanent teeth. At the cervical and central part of the fossa and pit, 
they are nearly horizontal.

Submicroscopic structures The enamel rods have an average diameter of 4 microns. 
The  diameter of the rod increases from the dentino-enamel junction towards the outer 
surface of  enamel in a ratio of about 1 : 2. The number of enamel rods has been estimated 
as ranging  from 5 million in the lower lateral incisor to 12 million in the upper first  molar. 
The  rods are  larger at cusp tips and shorter at the cervical region.

The head or body of the enamel rod is the broadest part and is about 5 microns wide, and 
the elongated thinner portion called as tail is about 1 micron wide. The rod, including both 
head and tail, is about 9 microns long.

In cross-section, the enamel rods appear as round, hexagonal or oval. Recent studies with 
electron microscope have shown that a more common pattern of enamel rods in cross 
section looks like a keyhole or paddle-shaped prism.


B. Rods Sheath


Under light microscope, a distinct thin layer is seen peripheral to the rods. It has a different 
refractive index, stains darker and is more acid-resistant than the rod. It is less calcified and 
contains more organic substance like enamel protein. This layer is known as rod sheath.

rod sheath




C. Inter-Rod Substances


Light microscope revealed that the rods are cemented together by inter-rod substance, 
which has slightly higher refractive index than the rods. The crystals are arranged in a 
different direction in the inter-rod region.


4) Other Structures


A) Hunter-Schreger Bands


Hunter-Schreger Bands



These are alternating dark and light bands which are best visualized in longitudinal ground 
section under oblique reflected light. 

It is produced solely by changes in the rod direction. 

The light bands are referred to as diazones and the dark bands are called as parazones. 

The angle between the diazones and parazones is approximately of 40 degrees

B) Incremental Lines of Retzius

Incremental lines of retzius


These are rhythmic successive apposition of layers of enamel during formation of the 
crown. 

When a ground section of a tooth is seen under a light microscope, concentric brown lines 
are seen in the enamel. These are called incremental lines of Retzius or Striae of Retzius. 

The incremental lines (striae) of Retzius are more frequently seen in permanent teeth and 
less frequently in deciduous teeth and prenatal enamel.

C) Structureless Outer Enamel Layer

30 microns thick found most commonly towards the cervical area and less often on cusp 
tips. 

This structure less layer is called prism less enamel and found in all deciduous teeth and in 
70 percent of the permanent teeth.

D) Perikymata

wave-like, transverse grooves. They are shallow furrows and most probably the external 
manifestation of incremental lines of Retzius. They are continuous around a tooth and 
usually lie parallel to each other and to the cemento-enamel junction.

Perikymata


E) Enamel Rods Ends

The enamel rod ends are concave and vary in depth and shape. They may contribute to the 
adherence of plaque material with a resultant caries attack, especially in young people.

Enamel Rods Ends


F) Enamel Lamellae

Enamel lamellae are very thin, leaf-like structures, sometimes visible to naked eye. They 
extend from the enamel surface towards the dentino-enamel junction, rarely extending into 
dentin. The enamel lamellae contain mostly organic material.

Enamel lamellae can be differentiated into three types:

Type A lamellae composed of poorly calcified rod segment.
Type B lamellae composed of degenerated cells.
Type C lamellae arising in erupted teeth where the cracks are filled with organic matter and 
debris from saliva.

Type A is restricted to enamel and type B and C may reach the dentin

enamel lamellae

G) Enamel Cracks

enamel cracks


Narrow, fissure-like structures that are present on almost all surfaces. They are actually the 
outer edges of enamel lamellae. They originate from dentino-enamel junction and run at 
right angles to it.

H) Neonatal Lines

In deciduous teeth, the enamel develops partly before and partly after birth. The line or 
boundary between the two portions of enamel in deciduous teeth is known as neonatal line 
or neonatal ring. It appears due to the abrupt change in the environment and nutrition of the 
newborn (infants). It is an accentuated incremental line of Retzius.

Neonatal Lines


I) Nasmyth’s Membrane (Primary Enamel Cuticle)

Nonmineralized usually found between the epithelium of dentogingival junction and the 
enamel surface. It is formed by an accumulation of basal lamina material produced by the 
junctional epithelium of the dentogingival junction. The final act of the ameloblast cell is 
secretion of a layer covering the end of the enamel rod. 

Nasmyth’s Membrane (Primary Enamel Cuticle)


J) Pellicle or Salivary Pellicle

Pellicle or Salivary Pellicle


After tooth is cleaned, salivary proteins and glycoproteins having strong affinity for enamel 
get adsorbed to the enamel surface very quickly and form a very thin layer called the 
salivary pellicle.

K) Enamel Tufts

Enamel Tufts


Enamel tufts are hypocalcifed enamel rods and interprismatic substance that originates at 
the dentinoenamel junction and extends into enamel for about one-third to one-fifth of its 
total thickness. They are known as enamel tufts because they look like tufts of grass 
projecting into enamel.

L) Dentino-Enamel Junction

Dentino-Enamel Junction


The dentinoenamel junction is a scalloped interface between the enamel and dentin. Dentin 
has pitted surface, which supports the enamel. Small curved projections of enamel fit into 
small concavities of the dentin.

M) Enamel Spindle

Enamel Spindle


Enamel Spindle


Odontoblastic processes sometimes cross the dentinoenamel junction and get entrapped in 
the enamel matrix. Since mostly they are thickened at their end they have been termed as 
enamel spindles. They may serve as pain receptors, thereby explaining the enamel 
sensitivity experienced by some patients during cavity preparation.

N) Gnarled Enamel

Gnarled Enamel


The enamel rods at the cuspal and incisal region appear intertwined, twisted and inter 
twisted, and are more irregular. Such kind of optical appearance of enamel is called as 
gnarled enamel. They are more so at the cuspal region than incisal region.

O) Enamel Droplets or Enamel Perals

enamel droplets or enamel pearls


Occasionally, the cells of the epithelial root sheath remain adherent to the dentin surface, 
they may differentiate into functioning ameloblasts and form small round islands of enamel. 
Such droplets of enamel are called enamel pearls. They may be found near or in the 
bifurcation or trifurcation of the roots of permanent molars.

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